Cultivation strategies for growth of uncultivated bacteria

Evaluation of Effective Strategies for Cultivation of Acidithiobacillus Thiooxidans as Cement-Degrading Bacteria

Are Uncultivated Bacteria Really Uncultivable?

Many strategies have been used to increase the number of bacterial cells that can be grown from environmental samples but cultivation efficiency remains a challenge for microbial ecologists. The difficulty of cultivating a fraction of bacteria in environmental samples can be classified into two non-exclusive categories. Bacterial taxa with no cultivated representatives for which appropriate lab...

Cultivation of a Synergistetes strain representing a previously uncultivated lineage

Subgingival plaque samples obtained from human subjects with periodontitis, shown to include previously uncultivable members of the phylum Synergistetes, were used to inoculate Cooked Meat Medium (CMM). The presence of Cluster A (uncultivable) Synergistetes was monitored by fluorescent in situ hybridization (FISH) and quantitative PCR (Q-PCR). Cluster A Synergistetes were found to grow in CMM i...

Microscopy studies on uncultivated magnetotactic bacteria

Magnetotactic bacteria are characterized by the magnetosome, a membrane-bound, nano-sized organelle containing a magnetic crystal. This organelle is believed to help the bacteria to navigate along the geomagnetic field lines and find a preferred habitat for survival. The magnetosome is a biomineral structure highly controlled by the bacteria, composed of either magnetite (Fe3O4) or greigite (Fe...

Preservation of Uncultivated Microbial Cells for Single Cell Genomics and Cultivation in Future

Microbes have conventionally been studied in pure culture obtained on culture media in the laboratory. With very limited media options at the disposal of a microbiologist as compared to the vast diversity of microbes that are known to exist, it is not surprising that only about 1-10% of them have been cultivated so far [1]. However, since microbes are the key components of every process on the ...